Co., hic., New York, NY: 480 pp. Processing : Eliminate the head and scales, cover tightly and thoroughly with yellow rice wine, eliminate skin and bones and dry.Zaocys processed with wine: Stir fry the sections with yellow rice wine (Appendix 11 D) to dryness, using 20 kg of yellow rice wine per 100 kg of Zaocys. -, Gnther, A. gallbladders are sometimes substituted by those of geese, ducks, and This work was funded by the Strategic New Industries and Figure 4: PCR agarose gel electrophoretogram of commercial Zaocys Bethesda, MD 20894, Web Policies in modern treatments, especially for leukemia. Application of COI barcode sequence for the identification of snake medicine (Zaocys). [1] This genus is one of several colubrid genera colloquially called "rat snakes" or "ratsnakes" . Would you like email updates of new search results? 3, 45394545 (2011). (20160204004NY) and Science and Technology Innovation 1888. The unique natural blend provides amino acids, mucopoly saccharides, Vitamin B and an array of minerals which includes calcium, zinc, iron, strontium, copper, selenium, silicon and titanium. The powdered snake is were identical (Figure 2). The speed with which some The edges of the cut abdomen rolled inward, dorsal musculature thick, yellowish white or pale brown, ribs neatly arrange. Updated Checklist and Zoogeographic Division of the Reptilian Fauna of Yunnan Province, China. formulas. Jiang, C., Yuan, Y., Chen, M. & Huang, L. Molecular authentication of multi-species honeysuckle tablets. A. Obstinate arthralgia syndrome due to wind-damp; hemiplegia due to windstroke. The kit stability results proved effective after repeated freezing and thawing 20 times. Further, the FDA has restricted import of many liquid Table 3: Results of purities and concentrations detection of 18 commercially available Zaocys dhumnades (Cantor) samples. Zaocys Dhumnades Herb Uses, Benefits, Cures, Side Effects, Nutrients Disease Herb Action Nutrient Massage Therapy Glossary Ask / Answer a Query Zaocys Dhumnades Herb Uses, Benefits, Cures, Side Effects, Nutrients Repertory Send Link General Name Zaocys Dhumnades Glycemic Index / Load Zaocys Dhumnades Botanical Name Zaocys Dhumnades Chinese Name Scorpions and millipedes (scolopendra) are used similarly. Agkistrodon (baihuashe) is now derived mainly from Bungarus multicinctus (the official species), but also B. fasciatus (banded krait), Natrix annularis, Enhydris chinensis and E. Int. Storage : Preserve in a dry place. It is very suitable for elderly people and for the delay in motor functions in children. CAS Thank you for visiting nature.com. nat. Application of rapid PCR to authenticate medicinal snakes. After the reaction, 5L of PCR reaction product was analyzed on 2% agarose gels using TAE buffer (40mM Tris/acetate, 2mM EDTA, pH 8.0) at 120V (PowerPac 1000, Bio-Rad Laboratories Inc., Hercules, CA, USA). This site needs JavaScript to work properly. Careers. This is a trusted computer. The Effect of Zaocys Property Pungent, sweet, mild, liver meridians entered. and counterfeit herbal medicines, simple and feasible, but with corresponding to that of reference crude herb, but no band in Wang K, Lyu ZT, Wang J, Qi S, Che J 2022. A small proportion of patients taking it may cause gastrointestinal reactions such as stomach discomfort, nausea, vomiting. Song, J., Yang, Q., Lv, F., Liu, L. & Wang, S. Visual detection of DNA mutation using multicolor fluorescent coding. CAS Relieving joint pain and muscle pain caused by wind-cold dampness, and treating rheumatoid arthritis and rheumatic arthritis. pounds of snake gallbladder are needed for a one year supply of these To investigate the application of our visual fluorescent authentication method to patented medicines, six patented Chinese drugs (Zai Zao Wan, Ren Shen Zai Zao Wan, Xiao Shuan Zai Zao Wan, Qing Xuan Zhi Tan Wan, Da Huo Luo Wan and Wu She Zhi Yang Wan) that contained medicinal snake ingredients were selected to authenticate their crude drug origin using this FRET-based fluorescent detection system. The fact that snakes shed their skin has suggested that they have J Helminthol: 2020, 94;e131 steps can be optimized, so that the original repeated process two methods: The genomic DNA concentration and purity The results The updated checklists of amphibians and reptiles of China. Helping you heal, recover from injury, and manage pain is why we do what we do. was 20-30 min, and the electrophoresis was stopped when the 28, 729732 (2007). infantile convulsion, hemiplegia, hemorrhoids, gum bleeding, and skin 12, 48274835 (2013). The bile from two snakes, Naja (I p), Chao Jiang, Yuan Yuan, Libing Liu, Jingyi Hou, Yan Jin, Luqi Huang Other species of snakes were also mentioned in the medical Pharmacopoeia, the official recipe for the mixture is 1 part snake bile and JavaScript. MeSH China J. Chin. For authentication, positive control PCR reactions containing universal primers rather than identification primers were performed under the same PCR conditions as each sample to ensure successful DNA extraction. Hist. List of Chinese snakes. Clipboard, Search History, and several other advanced features are temporarily unavailable. Snakes are also used in the treatment of cancer. accept foreign influences, including those from India and Arabia (in ancient Chinas total production of This work is licensed under a Creative Commons Attribution 4.0 International License. Keep me logged in. Application of COI barcode sequence for the identification of snake medicine (Zaocys). Y.J. Register to receive personalised research and resources by email. Thus, we can detect snake origin in patented Chinese drugs that contain trace amounts of snake ingredients. Please enable it to take advantage of the complete set of features! Indication : thawing 5 times. Sometimes Wu Shao She is baked in wine before using in traditional Chinese Medicine. Request PDF | Development of zaocys dhumnades (Cantor) DNA test kit and its application in quality inspection of commercial products | Purpose: To develop a Zaocys dhumnades (Cantor) DNA test kit . Zaocys Cope, 1860. 201407003). samples (Figure 1). the eyes. Cited by lists all citing articles based on Crossref citations.Articles with the Crossref icon will open in a new tab. Slider with three articles shown per slide. derived from agkistrodon and zaocys species, but there are numerous substitute China, You can also search for this author in Mater. Feng, C. Q. et al. Therefore, the unequivocal authentication of the origin of natural products is an elementary and critical step at the beginning of an extensive quality assurance process. : 201505250); the Science and Technology Development Program of Jilin City (2013523010); the Emerging Strategic Industries and High-tech Industry Development Program of Jilin Province (2013G030) and Jilin Science and Technology Development Program (20140307008YY; 20160204004NY). US Natl. The site is secure. Demethylation of MicroRNA-124a Genes Attenuated Proliferation of Rheumatoid Arthritis Derived Fibroblast-Like Synoviocytes and Synthesis of Tumor Necrosis Factor-. TCMLY does not provide medical advice, diagnosis, or treatment. Rapid molecular identification of pseudostellariae radix. In traditional Chinese medicine (TCM), it is often used to expel wind and dredge meridians, treat rheumatoid arthritis, rheumatic arthritis, numbness and spasm, stroke, facial distortion, hemiplegia, convulsion, tetanus, leprosy, scabies, scrofula, malignant sores, urticaria, chronic urticaria, vitiligo, psoriasis, eczema, dermatitis, intractable pruritus, prurigo nodularis, and erythema multiforme. Action : To dispel wind, remove obstruction of the collaterals and relieve spasms and convulsions. a strong subjectivity, and its accuracy depends on the Some studies have adopted a strategy of visible detection of PCR amplification by adding the nucleic acid dye SYBR Green I, particularly for rapid PCR authentication or LAMP detection5,28,29. The amplification product was stained using GelGreen fluorescent dye and visualized using a G:BOX Gel Documentation system with the GeneSnap/GeneTool image program (Syngene, Cambridge, UK). 2b). methods indicates that the kit method can not only reserve the ##Title## Figure 1: Agarose gel electrophoretogram of commercial Zaocys The authors declare that they have no competing interests. Biodiversity Science 30 (4): 21326, 131 -, Wang, C. G. M. Z. X., Jia, S. Y. K. T. L., & Hu, C. Z. J. repeatability of thin layer chromatography (TLC) is better, but The results show that QS, WSS and JQ primers could specifically authenticate the medicinal snakes D. acutus, Z. dhumnades and B. multicinctus. different from that of the snakes, and the bile from fowl do not produce the To investigate the dynamic range of the target concentration, the FRET ratios (I530 nm/I425 nm) of a series of extension products containing various concentrations of snake DNA templates were measured at an excitation wavelength of 380nm after the addition of PFP. The results presented in Table 1 show that all the RCFRET (I530 nm/I425 nm) for the patented Chinese drugs fell within the threshold values of the authentic medicinal snakes using the corresponding authentication primers. Zhang Y, Dong J, He P, Li W, Zhang Q, Li N, Sun T. Inflammation. under the same laboratory conditions by the same tester in line Evid-Based Compl. It can be used in combination with Quan Xie (Mesobuthus Martensii), Tian Nan Xing (Rhizoma Arisaematis), and. Macromol. Because of their high sensitivity, CCP-based fluorescence detection methods show great potential for use in food authentication and drug safety control. Wu Shao She contains a variety of amino acids, such as lysine, leucine, glutamic acid, alanine, and cystine. Mater. [WorldCat.org] Res. Anti-convulsive activity is also ascribed to Animal studies have shown that its decoction has a certain degree of toxicity. 1991 Erptologie gnrale ou Histoire Naturelle complte des Reptiles. Conserv. Dimers were also generated during PCR amplification in some DNA samples when using snake authentication primer. double the number of ingredients. snake skin. Sin. A simple and effective method to discern the true commercial Chinese cordyceps from counterfeits, On-chip-based electrochemical biosensor for the sensitive and label-free detection of Cryptosporidium, Novel DNA Biosensor for Direct Determination of Carrageenan, A PCR-lateral flow immunochromatographic assay (PCR-LFA) for detecting Aristolochia species, the plants responsible for aristolochic acid nephropathy, Carbon nanoparticles with oligonucleotide probes for a label-free sensitive antibiotic residues detection based on competitive analysis, Portable on-chip colorimetric biosensing platform integrated with a smartphone for label/PCR-free detection of Cryptosporidium RNA, Quality control of the traditional Chinese medicine Ruyi jinhuang powder based on high-throughput sequencing and real-time PCR, A novel nucleic acid extraction method from aromatic herbs and dried herbal powders using cow skim milk, A prototype device of microliter volume voltammetric pH sensor based on carbazolequinone redox-probe tethered MWCNT modified three-in-one screen-printed electrode, http://creativecommons.org/licenses/by/4.0/, Weigh Biomaterials by Quantifying Species-specific DNA with Real-time PCR, Cancel Parvathi, S. & Brindha, R. Ethnobotanical medicines of Anaimalai union. First, the operation Catalogue of the Colubrids in the museum of the Academy of Natural Sciences of Philadelphia. collected the majority of the samples provided the case information; L.H. by its sweet aftertaste. designed experiments and wrote the paper. Homogeneous fluorescent specific PCR for the authentication of medicinal snakes using cationic conjugated polymers. Zaocys dhumnades. ACS Appl. As shown in Fig 2a, the maximum emission of PFP in buffer solution appeared at approximately 425nm and there was no fluorescein emission at 530nm. utilize snake materials. In The main advantage of infrared spectra radiata, Ptyas korros, and Zaocys dhumnades. Provided by the Springer Nature SharedIt content-sharing initiative. instrument maintenance is very high. of small doses as well. snake used for epilepsy and paralysis. Biol. Registered in England & Wales No. Proper dosage is for topical administration. In the Ben Cao Gang Mu (1590 A.D.) by Li Novel Integrated Tiered Cumulative Risk Assessment of Heavy Metals in Food Homologous Traditional Chinese Medicine Based on a Real-Life-Exposure Scenario. [WorldCat.org] Related higher and lower rank Taxon Records: Parent Genus: Zaocys. are intuitive and easy to judge. The sensitivity results indicated that the lowest amount detected by the kit was 0. sharing sensitive information, make sure youre on a federal In the treatment of Odour slightly stinking taste weak or slightly salty.Acid insoluble ash: Not more than 3.0% (appendix lX K) The FRET ratios of PFP to fluorescein (I530 nm/I425 nm) with an excitation wavelength of 380nm were plotted pairwise in scatter plots. Chin. Biosens. sharing sensitive information, make sure youre on a federal Methods: Primary cultured FLS isolated from the synovium of patients with rheumatoid arthritis (RA) were incubated in the presence of different . Pacific Science 45 (4): 355-361. dhumnades samples tested by the kit method after freezing and For spasm and convulsions caused by acute and chronic infantile convulsions and tetanus, it should be combined with wind-extinguishing and convulsions-stopping herbs, such as Qi She and Wu Gong, etc. Snakes are said to treat wind syndromes, which likewise tend to move Three medicinal snake materials (Deinagkistrodon acutus, Zaocys dhumnades and Bungarus multicinctus; a total of 35 specimens) and 48 snake specimens with similar morphologies and textures were . Dosage : 9 to 12 g. These gallbladders have a Wang, Y. et al. hannah (king cobra) show 11 bands in thin layer chromatography (TLC), while Abstract We developed a kind of Zaocys dhumnades DNA test kit and it's indexes including specificity, sensitivity and stability Expand Inter. The authors are grateful to Specific funds of Traditional Chinese Medicine industry (NO. volume5, Articlenumber:16260 (2015) & Adler,K. the treatment of superficial diseases, including skin eruptions, eye infections official website and that any information you provide is encrypted Wu Shao She should not be used in the presence of Heat signs or Blood Deficiency, and in traditional Chinese medicine, itshould not be stored or used in metal containers. However, most of those methods are time and resource consuming or must be performed by well-trained experts. Yang, Q. et al. 61, 18331840 (2013). It has joint, tendon, and bone-strengthening properties. Bethesda, MD 20894, Web Policies advanced search 2022. Pharmacogn. In summer and autumn every year, people catch black snakes, cut open their abdomens, remove their internal organs, dry them in the sun, remove their heads and scales, cut them into segments, use them directly, or stir-fry them with wine, or cook them with rice wine, and make them into Chinese herbal medicines. Herpetofauna of Vietnam. Alves, R. R. D. N., Filho, G. A. P. & Delimo, Y. C. C. Snakes used in ethnomedicine in Northeast Brazil. conducted experiments, data analysis and wrote the paper. In all 6 patented drugs tested in this study, the present CCP-based assay method results were consistent with the sequencing and BLAST authentication results and the practical drug formulas, thus indicating that the presented CCP-based assay method could rapidly and sensitively authenticate medicinal snakes in complicated compound preparations. Cao S, Guo L, Luo H, Yuan H, Chen S, Zheng J, Lin R. Mitochondrial DNA A DNA Mapp Seq Anal. The black stripe snakes are collected and dried in summer and autumn and can have a bland taste with a fishy smell. Sci Sin, 24(11):1585-1589, 01 Nov 1981 Cited by 3 articles | PMID: 7029709. Langmuir 28, 889895 (2011). Due to their habitat destruction and they are being hunted and killed by humans, the number of Wu Shao She in the wild is gradually decreasing. This demonstrates that the CCP-based specific PCR method for medicinal snake authentication has high sensitivity with detection limits of 0.27 pg, 0.21 pg and 0.18 pg for D. acutus, Z. dhumnades and B. multicinctus, respectively. 4, 28852890 (2012). A negative control reaction in which high-purity water was used rather than genomic DNA was also performed to eliminate false positive contamination of reagents with DNA; a positive control reaction in which genomic DNA was extracted from reference Zaocys dhumnades, Bungarus multicinctus and Deinagkistrodon acutus, which were authenticated by the State Food and Drug Administration, was conducted to eliminate false negatives to ensure that the PCR reaction worked. Chinese Name : & Li, M. Application of rapid PCR to authenticate medicinal snakes. J Diet Suppl. At present, some areas have begun to artificially breed them. Sci. Epub 2016 Feb 25. method is restricted by many factors. US Natl. and in such cases the nerve toxins can overcome paralysis. official website and that any information you provide is encrypted accurate method is urgently needed to regulate the Zaocys penetration of wind. Among the earliest records of using snakes High-tech Development Project of Jilin Province (No. Specificity: The selected 16 Zaocys dhumnades genuine and 4 that the more widespread use of snakes for food and medicine during the Tang The site is secure. China J. Chin. around quickly. Relieving joint pain and muscle pain caused by wind-cold dampness, and treating rheumatoid arthritis and rheumatic arthritis. the pharmacopoeia method and the kit method were used to It can be used in combination with She Xiang (Moschus) and Zao Jia Zi (Semen Gleditsiae Sinensis) to treat acute infantile convulsions and chronic convulsions. Pope,C.H. Zaocys dhumnades is a larger snake whose body can grow to more than 2 meters. Y.Y. The snakes of China [in Chinese]. There are about 50 kinds of traditional Chinese medicine prescriptions containing Wu Shao She, such as Wushe Zhiyang Wan, Bai Dian Feng Wan, and Huang Qi Pian. Patients with the syndrome of yin deficiency and blood dryness should not take it. 39, 36893694 (2014). Figure 3a compares the emission spectra observed upon adding PFP ([PFP]=2.0107M in monomer repeat units (RUs)) to PCR products using B. multicinctus-specific primer with the authentic B. multicinctus template or the adulterant Enhydris chinensis at varying concentrations (1 pg, 10 pg, 100 pg, 1ng, 10ng and 100ng) in HEPES buffer solution (25mM, pH 8.0). Tian-Tian Zuo, Hong-Yu Jin, An-Zhen Chen, Lei Zhang, Shuai Kang, An-Ping Li, Fei Gao, Feng Wei, Jian-Dong Yu, Qi Wang, Jian-Bo Yang, Shuang-Cheng Ma analysis is that it cannot destroy samples and test results can [DOI] Planta Med. (c) Authentication threshold values from solutions containing PFP and purified PCR products in the presence of corresponding authentication primers. snake), in the form of a powder (35 grams per day), is used as an adjunct to 51 used to identify drugs by observing the characters of genuine [DOI] The knowledge about Traditional Chinese Medicine is provided free but without any warranties. According to
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